If you've washed a spot more times than you can count and your puppy continues to eliminate there, it's now more of a habit for your puppy than anything else.
The first thing you can do is to use a repellent. Just spray the spot and see how your puppy reacts to it. It may very well solve your problem.
The second solution is to supervise your puppy very closely. Maybe you thought she was completely housetrained and allowed her too much freedom. Go back a couple of steps. Keeping her in her domain one more week may also help break the habit and reinforce the use ofher elimination zone or toilet stop.
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